***** Running buildChIPprofile (SeqCode_v1.0) by Enrique Blanco (2020) ***** ***** GitHub source code: https://github.com/eblancoga/seqcode ***** ***** SeqCode homepage at: http://ldicrocelab.crg.es ***** > 0. Running on Wed Jun 9 14:40:57 2021 > 1. Request Memory to Operating System Loading the R color schema > 2. Reading Chromosome Sizes Dictionary (hash): chr8 | 7 chr9 | 8 chr10 | 9 chr11 | 10 chr12 | 11 chrX | 20 chr13 | 12 chrY | 21 chr14 | 13 chr15 | 14 chr16 | 15 chr17 | 16 chr18 | 17 chr19 | 18 chr1 | 0 chr2 | 1 chr3 | 2 chr4 | 3 chrM | 19 chr5 | 4 chr6 | 5 chr7 | 6 Chromosome sizes: 0 | 197195432 1 | 181748087 2 | 159599783 3 | 155630120 4 | 152537259 5 | 149517037 6 | 152524553 7 | 131738871 8 | 124076172 9 | 129993255 10 | 121843856 11 | 121257530 12 | 120284312 13 | 125194864 14 | 103494974 15 | 98319150 16 | 95272651 17 | 90772031 18 | 61342430 19 | 16299 20 | 166650296 21 | 15902555 Size was successfully acquired for 22 chromosomes > 3. Prepare the Output Folder The prefix folder ./ exists The prefix folder ./catalina/ exists The prefix folder ./catalina/results/ exists Creating the output folder ./catalina/results/Mel18_mESC_profile Filename for the BedGraph profile ./catalina/results/Mel18_mESC_profile/Mel18_mESC.bedgraph > 4. Processing the BAM/SAM file samples/mouse/Mel18_mESC.bam: 586.77 Mb Processing Single-end sequencing reads Reading:  [ 0 lines] [ 100000 lines] [ 200000 lines] [ 300000 lines] [ 400000 lines] [ 500000 lines] [ 600000 lines] [ 700000 lines] [ 800000 lines] [ 900000 lines] [ 1000000 lines] [ 1100000 lines] [ 1200000 lines] [ 1300000 lines] [ 1400000 lines] [ 1500000 lines] [ 1600000 lines] [ 1700000 lines] [ 1800000 lines] [ 1900000 lines] [ 2000000 lines] [ 2100000 lines] [ 2200000 lines] [ 2300000 lines] [ 2400000 lines] [ 2500000 lines] [ 2600000 lines] [ 2700000 lines] [ 2800000 lines] [ 2900000 lines] [ 3000000 lines] [ 3100000 lines] [ 3200000 lines] [ 3300000 lines] [ 3400000 lines] [ 3500000 lines] [ 3600000 lines] [ 3700000 lines] [ 3800000 lines] [ 3900000 lines] [ 4000000 lines] [ 4100000 lines] [ 4200000 lines] [ 4300000 lines] [ 4400000 lines] [ 4500000 lines] [ 4600000 lines] [ 4700000 lines] [ 4800000 lines] [ 4900000 lines] [ 5000000 lines] [ 5100000 lines] [ 5200000 lines] [ 5300000 lines] [ 5400000 lines] [ 5500000 lines] [ 5600000 lines] [ 5700000 lines] [ 5800000 lines] [ 5900000 lines] [ 6000000 lines] [ 6100000 lines] [ 6200000 lines] [ 6300000 lines] [ 6400000 lines] [ 6500000 lines] [ 6600000 lines] [ 6700000 lines] [ 6800000 lines] [ 6900000 lines] [ 7000000 lines] [ 7100000 lines] [ 7200000 lines] [ 7300000 lines] [ 7400000 lines] [ 7500000 lines] [ 7600000 lines] [ 7700000 lines] [ 7800000 lines] [ 7900000 lines] [ 8000000 lines] [ 8100000 lines] [ 8200000 lines] [ 8300000 lines] [ 8400000 lines] [ 8500000 lines] [ 8600000 lines] [ 8700000 lines] [ 8800000 lines] [ 8900000 lines] [ 9000000 lines] [ 9100000 lines] [ 9200000 lines] [ 9300000 lines] [ 9400000 lines] [ 9500000 lines] [ 9600000 lines] [ 9700000 lines] [ 9800000 lines] [ 9900000 lines] [ 10000000 lines] [ 10100000 lines] [ 10200000 lines] [ 10300000 lines] [ 10400000 lines] [ 10500000 lines] [ 10600000 lines] [ 10700000 lines] [ 10800000 lines] [ 10900000 lines] [ 11000000 lines] [ 11100000 lines] [ 11200000 lines] [ 11300000 lines] [ 11400000 lines] [ 11500000 lines] [ 11600000 lines] [ 11700000 lines] [ 11800000 lines] [ 11900000 lines] [ 12000000 lines] [ 12100000 lines] [ 12200000 lines] [ 12300000 lines] [ 12400000 lines] [ 12500000 lines] [ 12600000 lines] [ 12700000 lines] [ 12800000 lines] [ 12900000 lines] [ 13000000 lines] [ 13100000 lines] [ 13200000 lines] [ 13300000 lines] [ 13400000 lines] [ 13500000 lines] [ 13600000 lines] [ 13700000 lines] [ 13800000 lines] [ 13900000 lines] [ 14000000 lines] [ 14100000 lines] [ 14200000 lines] 14215505 reads of the SAM file processed 7110774 forward reads acquired 7105074 reverse reads acquired > 5. Building the BedGraph profile Processing chromosome chr8 (7) - 131738871 Processing chromosome chr9 (8) - 124076172 Processing chromosome chr10 (9) - 129993255 Processing chromosome chr11 (10) - 121843856 Processing chromosome chr12 (11) - 121257530 Processing chromosome chrX (20) - 166650296 Processing chromosome chr13 (12) - 120284312 Processing chromosome chrY (21) - 15902555 Processing chromosome chr14 (13) - 125194864 Processing chromosome chr15 (14) - 103494974 Processing chromosome chr16 (15) - 98319150 Processing chromosome chr17 (16) - 95272651 Processing chromosome chr18 (17) - 90772031 Processing chromosome chr19 (18) - 61342430 Processing chromosome chr1 (0) - 197195432 Processing chromosome chr2 (1) - 181748087 Processing chromosome chr3 (2) - 159599783 Processing chromosome chr4 (3) - 155630120 Processing chromosome chrM (19) - 16299 Processing chromosome chr5 (4) - 152537259 Processing chromosome chr6 (5) - 149517037 Processing chromosome chr7 (6) - 152524553 22 chromosomes sucessfully included into the BG profile Size of the resulting BG file (uncompressed): ./catalina/results/Mel18_mESC_profile/Mel18_mESC.bedgraph: 419.84 Mb Compressing the final BG profile gzip ./catalina/results/Mel18_mESC_profile/Mel18_mESC.bedgraph Size of the resulting BG file: ./catalina/results/Mel18_mESC_profile/Mel18_mESC.bedgraph.gz: 83.36 Mb __________________________________________________________________________ Memory usage: 109.67 Megabytes CPU time: 13.22 secs Total time: 40 secs (0.67 mins)