pdf("./catalina/results/RING1B_EwS_GENEplot_5000/PlotGENEprofile_RING1B_EwS_5000.pdf",bg="white",fg="black") c <- read.table ("./catalina/results/RING1B_EwS_GENEplot_5000/GENEprofile_RING1B_EwS_5000.txt") plot(c[,1],c[,2],xlab="GENE",ylab="RING1B_EwS normalized count of reads",main="GENE profile for RING1B_EwS (5000 bp, 25718321 reads)",axes=FALSE,pch=" ",col.axis="black",col.main="black",col.lab="black") polygon(c(-6000,-6000,16000,16000),c(0,max(c[,2])+1000,max(c[,2])+1000,0),col="white") lines(c,col="black",lwd=8) lines(c(0,0),c(0,max(c[,2])),lwd=1,lty=2,col="black") lines(c(10000,10000),c(0,max(c[,2])),lwd=1,lty=2,col="black") par(font = 2,col.axis="black",col.main="black",col.lab="black") axis(1,at=c(-5000,0,10000,15000),labels=c("-5000","TSS","TES","+5000"),col="black") axis(2,col="black") box(col="black") mtext(side=4,cex=0.5,font=2,"SeqCode by E. Blanco [Wed Jun 9 15:47:21 2021]") plot(0:10, type = "n", xaxt="n", yaxt="n", bty="n", xlab = "", ylab = "") text(5.25,10,"Figure legend. Average distribution of ChIPseq reads along a uniform gene model.",font=2,cex=0.75) text(5.35,9.5,"This plot is generated by counting the number of reads along this region for each gene and",font=1,cex=0.75) text(5.4,9,"averaging this value for the number of genes and the number of mapped reads (in millions).",font=1,cex=0.75) text(5.1,8.5,"The X-axis represents the metagene and the flanking region in which the counts were",font=1,cex=0.75) text(5.225,8,"calculated for each gene. The Y-axis represents the intensity of the average ChIP signal",font=1,cex=0.75) text(5.25,7.5,"normalized by the number of reads of the sample. TSS is the Transcription Start Site and",font=1,cex=0.75) text(2.375,7,"TES is the Transcription End Site.",font=1,cex=0.75) text(5,5.5,"ChIPseq experiment:",font=1,cex=0.75) text(5,5,"RING1B_EwS",font=3,cex=0.75) text(5,4.5,"Number of reads:",font=1,cex=0.75) text(5,4,"25718321",font=3,cex=0.75) text(5,3.5,"Number of genes:",font=1,cex=0.75) text(5,3,"20248",font=3,cex=0.75) text(5,2.5,"Flanking sequence:",font=1,cex=0.75) text(5,2,"5000",font=3,cex=0.75) text(5,1.5,"RefSeq transcripts:",font=1,cex=0.75) text(5,1,"78106",font=3,cex=0.75) text(5,0.5,"Window factor:",font=1,cex=0.75) text(5,0,"10",font=3,cex=0.75) mtext(side=4,cex=0.5,font=2,"SeqCode by E. Blanco [Wed Jun 9 15:47:21 2021]") dev.off()